Sperm surface protein

Obviously this influences the quality of such a specimen as epididymal sperm may not be fully matured and the amount of sperm collected is not sufficient for proper membrane subfractionation studies. Beyond the purification of APMs another good method of subcellular membrane fractionation that has become increasingly popular in recent years is the isolation of lipid rafts also known as microdomains or detergent resistant membranes, DRMs. Integrated resource of protein families, domains and functional sites More National Institutes of Health. Lectins can bind to specific sugar residues at the extracellular domain of integral membrane proteins and some marker lectins exclusively bind to the sperm plasma membrane. This is especially relevant for studying proteins involved in zona recognition.

In order to identify many or all separated proteins from a 2D gel it is necessary to excise multiple gel plugs and subject them to this workflow and this can be assisted by robotic solutions. Notwithstanding these concerns these are still useful techniques and there are many ways to reduce contamination Dormeyer et al. Within the scope of this review the shortcomings of membrane protein solubilization for 2DE is well-illustrated in a recently published proteomic study on human sperm. Initial iodide concentration of the sperm. Overall much more reliable surface membrane protein samples can be obtained from these species compared with either the human or the mouse.

Other studies have also revealed a similar number of proteins Pixton et al. Kyoto Encyclopedia of Genes and Genomes More Capacitation dependent activation of tyrosine phosphorylation generates two sperm head plasma membrane proteins with high primary binding affinity for the zona pellucida. This page was last edited on 8 November , at

Although informative ideally one would use a strategy to localize the membrane proteins even more precisely and define the purity of the fractions. Human spermatozoa contain multiple targets for protein S-nitrosylation: Glycolipid migration from the apical to the equatorial subdomains of the sperm head plasma membrane precedes the acrosome reaction. The names of proteins and their respective classes in the three clusters. In this review, we have given an overview of novel proteomic methodologies to explore the plasma membrane in sperm at fertilization and have argued for a greater focus on the cell surface proteome to achieve greater biological understanding. You are using a version of browser that may not display all the features of this website.