Introduction Artificial insemination is considered to be an in vivo assay of spermatozoa survival and is the most definitive test of sperm function. Because the causes of infertility in male dogs are so variable, diagnosing the precise cause of the problem can be difficult. An accurate evaluation of the LH spike can give the veterinarian and breeder 4 to 5 days anticipation of the prime insemination dates and reasonable confidence as to the maximum chance of conception. If resistance is encountered, the rod should be gently twisted or withdrawn a short distance, then readvanced. The penis and prepuce will be examined for evidence of abnormality or disease, and the prostate will be assessed both by rectal and abdominal palpation. First and foremost before allowing a dog pregnancy, make sure you have properly protected your female dog and her potential litter by following these steps: The thawed spermatozoa has a maximum life span of 12 to 24 hours after insemination into the bitch.
CANINE SPERM COUNT & MOTILITY IN FROZEN SEMEN STUDY BY TEXAS A&M
The day on which the progesterone concentration initially entered the range of 4. Five replicate experiments were performed. Influence of ascorbic acid and glutathione antioxidants on frozen-thawed canine semen. Sperm Abnormalities in Dogs Book in. This is in contrast to other estrous cycles, such as the bovine estrous cycle, where SOD activity in oviductal fluid remains constant throughout [ 14 ].
It provides the best opportunity for successful pregnancy. There has been no trauma. Factors influencing the success rate of artificial insemination with frozen semen in the dog. There are many simple factors that can contribute to the reading of a low sperm count, like temperature of the microscope slide or use of medication. Male genital tract antioxidant enzymes:
Therefore, the average season or heat is 18 days, with ranges from days reported for normal, fertile bitches. LH testing is especially useful in bitches previously tested by progesterone assay that did not conceive to that showed irregular progesterone levels. This article has been cited by other articles in PMC. These results indicate that one optimally timed EIU insemination results in similar pregnancy rates to previous publications of one optimally timed, or two or more non-optimally timed inseminations using the Norwegian catheter. However, vaginal smearing does not allow prospective timing, other than giving information as to when other testing needs to begin. There is no room for error!! For frozen-thawed semen deposited into the uterus, an insemination dose of around 1.